Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells

2014 | journal article; research paper. A publication with affiliation to the University of Göttingen.

Jump to:Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells​
Honigmann, A.; Mueller, V.; Ta, H. ; Schoenle, A. ; Sezgin, E.; Hell, S.   & Eggeling, C. ​ (2014) 
Nature Communications5 art. 5412​.​ DOI: https://doi.org/10.1038/ncomms6412 

Documents & Media

document.pdf1.67 MBAdobe PDF

License

GRO License GRO License

Details

Authors
Honigmann, Alf; Mueller, Veronika; Ta, Haisen ; Schoenle, Andreas ; Sezgin, Erdinc; Hell, Stefan ; Eggeling, Christian 
Abstract
The interaction of lipids and proteins plays an important role in plasma membrane bioactivity, and much can be learned from their diffusion characteristics. Here we present the combination of super-resolution STED microscopy with scanning fluorescence correlation spectroscopy (scanning STED-FCS, sSTED-FCS) to characterize the spatial and temporal heterogeneity of lipid interactions. sSTED-FCS reveals transient molecular interaction hotspots for a fluorescent sphingolipid analogue. The interaction sites are smaller than 80nm in diameter and lipids are transiently trapped for several milliseconds in these areas. In comparison, newly developed fluorescent phospholipid and cholesterol analogues with improved phase-partitioning properties show more homogenous diffusion, independent of the preference for liquid-ordered or disordered membrane environments. Our results do not support the presence of nanodomains based on lipid-phase separation in the basal membrane of our cultured nonstimulated cells, and show that alternative interactions are responsible for the strong local trapping of our sphingolipid analogue.
Issue Date
2014
Journal
Nature Communications 
ISSN
2041-1723
Language
English

Reference

Citations


Social Media