Deficiency of UDP-galactose : N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId

2002 | journal article. A publication with affiliation to the University of Göttingen.

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​Deficiency of UDP-galactose : N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId​
Hansske, B.; Thiel, C.; Lubke, T.; Hasilik, M.; Honing, S.; Peters, V. & Heidemann, P. H. et al.​ (2002) 
Journal of Clinical Investigation109(6) pp. 725​-733​.​ DOI: https://doi.org/10.1172/JCI200214010 

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Authors
Hansske, B.; Thiel, C.; Lubke, T.; Hasilik, M.; Honing, S.; Peters, V.; Heidemann, P. H.; Hoffmann, Georg F.; Berger, E. G.; von Figura, Kurt; Korner, C.
Abstract
Deficiency of the Golgi enzyme UDP-Gal:N-acetylglucosamine beta-1,4-galactosyltransferase I (beta4GalT I) (E.C.2.4.1.38) causes a new congenital disorder of glycosylation (CDG), designated type IId (CDG-IId), a severe neurologic disease characterized by a hydrocephalus, myopathy, and blood-clotting defects. Analysis of oligosaccharides from serum transferrin by HPLC, mass spectrometry, and lectin binding revealed the loss of sialic acid and galactose residues. In skin fibroblasts and leukocytes, galactosyltransferase activity was reduced to 5% that of controls. In fibroblasts, a truncated polypeptide was detected that was about 12 kDa smaller in size than wild-type beta4GalT I and that failed to localize to the Golgi apparatus. Sequencing of the beta4GaIT I cDNA and gene revealed an insertion of a single nucleotide (1031-1032insC) leading to premature translation stop and loss of the C-terminal 50 amino acids of the enzyme. The patient was homozygous and his parents hererozygous for this mutation. Expression of a corresponding mutant cDNA in COS-7 cells led to the synthesis of a truncated, inactive polypeptide, which localized to the endoplasmic reticulum.
Issue Date
2002
Status
published
Publisher
Amer Soc Clinical Investigation Inc
Journal
Journal of Clinical Investigation 
ISSN
0021-9738

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