Fluorogenic probes for live-cell imaging of the cytoskeleton

2014 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Fluorogenic probes for live-cell imaging of the cytoskeleton​
Lukinavicius, G.; Reymond, L.; D'Este, E. ; Masharina, A.; Göttfert, F. ; Ta, H.   & Güther, A. et al.​ (2014) 
Nature Methods, 11(7) pp. 731​-733​.​ DOI: https://doi.org/10.1038/NMETH.2972 

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Authors
Lukinavicius, Grazvydas; Reymond, Luc; D'Este, Elisa ; Masharina, Anastasiya; Göttfert, Fabian ; Ta, Haisen ; Güther, Angelika; Fournier, Mathias; Rizzo, Stefano; Waldmann, Herbert; Blaukopf, Claudia; Sommer, Christoph; Gerlich, Daniel Wolfram; Arndt, Hans-Dieter; Hell, Stefan ; Johnsson, Kai
Abstract
We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.
Issue Date
2014
Journal
Nature Methods 
ISSN
1548-7091
eISSN
1548-7105
Language
English

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