Overexpression of bacterial γ-glutamylcysteine synthetase mediates changes in cadmium influx, allocation and detoxification in poplar

2015 | journal article. A publication with affiliation to the University of Göttingen.

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​Overexpression of bacterial γ-glutamylcysteine synthetase mediates changes in cadmium influx, allocation and detoxification in poplar​
He, J.; Li, H.; Ma, C.; Zhang, Y.; Polle, A. ; Rennenberg, H. & Cheng, X. et al.​ (2015) 
New Phytologist205(1) pp. 240​-254​.​ DOI: https://doi.org/10.1111/nph.13013 

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Authors
He, Jiali; Li, Hong; Ma, Chaofeng; Zhang, Yanli; Polle, Andrea ; Rennenberg, Heinz; Cheng, Xingqi; Luo, Zhi-Bin
Abstract
Overexpression of bacterial γ‐glutamylcysteine synthetase in the cytosol of Populus tremula × P. alba produces higher glutathione (GSH) concentrations in leaves, thereby indicating the potential for cadmium (Cd) phytoremediation. However, the net Cd2+ influx in association with H+/Ca2+, Cd tolerance, and the underlying molecular and physiological mechanisms are uncharacterized in these poplars. We assessed net Cd2+ influx, Cd tolerance and the transcriptional regulation of several genes involved in Cd2+ transport and detoxification in wild‐type and transgenic poplars. Poplars exhibited highest net Cd2+ influxes into roots at pH 5.5 and 0.1 mM Ca2+. Transgenics had higher Cd2+ uptake rates and elevated transcript levels of several genes involved in Cd2+ transport and detoxification compared with wild‐type poplars. Transgenics exhibited greater Cd accumulation in the aerial parts than wild‐type plants in response to Cd2+ exposure. Moreover, transgenic poplars had lower concentrations of O2˙− and H2O2; higher concentrations of total thiols, GSH and oxidized GSH in roots and/or leaves; and stimulated foliar GSH reductase activity compared with wild‐type plants. These results indicate that transgenics are more tolerant of 100 μM Cd2+ than wild‐type plants, probably due to the GSH‐mediated induction of the transcription of genes involved in Cd2+ transport and detoxification.
Issue Date
2015
Journal
New Phytologist 
Organization
Fakultät für Forstwissenschaften und Waldökologie ; Büsgen-Institut ; Abteilung Forstbotanik und Baumphysiologie 
ISSN
0028-646X
Language
English

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