p53's mitochondrial translocation and MOMP action is independent of Puma and Bax and severely disrupts mitochondrial membrane integrity

2008 | journal article. A publication with affiliation to the University of Göttingen.

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​Wolff, S., Erster, S., Palacios, G. & Moll, U. M. (2008). ​p53's mitochondrial translocation and MOMP action is independent of Puma and Bax and severely disrupts mitochondrial membrane integrity. Cell Research18(7), ​733​-744​. ​doi: https://doi.org/10.1038/cr.2008.62 

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Authors
Wolff, Sonja; Erster, Susan; Palacios, Gustavo; Moll, Ute M.
Abstract
p53's apoptotic program consists of transcription-dependent and transcription-independent pathways. In the latter, physical interactions between mitochondrial p53 and anti- and pro-apoptotic members of the Bcl2 family of mitochondrial permeability regulators are central. Using isogenic cell systems with defined deficiencies, we characterize in detail how mitochondrial p53 contributes to mitochondrial permeabilization, to what extent its action depends on other key Bcl2 family members and define its release activity. We show that mitochondrial p53 is highly efficient in inducing the release of soluble and insoluble apoptogenic factors by severely disrupting outer and inner mitochondrial membrane integrity. This action is associated with wild-type p53-induced oligomerization of Bax, Bak and VDAC and the formation of a stress-induced endogenous complex between p53 and cyclophilin D, normally located at the inner membrane. Tumor-derived p53 mutants are deficient in activating the Bax/Bak lipid pore. These actions are independent of Puma and Bax. Importantly, the latter distinguishes the mitochondrial from the cytosolic p53 death pathway.
Issue Date
2008
Status
published
Publisher
Nature Publishing Group
Journal
Cell Research 
ISSN
1001-0602
Sponsor
NCI NIH HHS [R01 CA060664, R01 CA060664-13]

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