Subcellular Targeting of VIP Boutons in Mouse Barrel Cortex is Layer-Dependent and not Restricted to Interneurons

journal article

Jump to: Cite & Linked | Documents & Media | Details | Version history

Cite this publication

Subcellular Targeting of VIP Boutons in Mouse Barrel Cortex is Layer-Dependent and not Restricted to Interneurons
Zhou, X. ; Rickmann, M. ; Hafner, G.   & Staiger, J. F.  (2017) 
Cerebral Cortex27(11) pp. 5353-5368.​

Documents & Media

bhx220.pdf8.72 MBAdobe PDF

License

Published Version

Attribution-NonCommercial 4.0 CC BY-NC 4.0

Details

Authors
Zhou, Xiaojuan ; Rickmann, Michael ; Hafner, Georg ; Staiger, Jochen F. 
Abstract
Neocortical vasoactive intestinal polypeptide (VIP) expressing cells are a diverse subpopulation of GABAergic interneurons issuing distinct axonal projections. They are known to inhibit other types of interneurons as well as excitatory principal neurons and possess a disinhibitory net effect in cortical circuits. In order to elucidate their targeting specificity, the output connectivity of VIP interneurons was studied at the subcellular level in barrel cortex of interneuron-specific Cre-driver mice, using pre- and postembedding electron microscopy. Systematically sampling VIP boutons across all layers, we found a substantial proportion of the innervated subcellular structures were dendrites (80%), with somata (13%), and spines (7%) being much less targeted. In layer VI, a high proportion of axosomatic synapses was found (39%). GABA-immunopositive ratio was quantified among the targets using statistically validated thresholds: only 37% of the dendrites, 7% of the spines, and 26% of the somata showed above-threshold immunogold labeling. For the main target structure "dendrite", a higher proportion of GABAergic subcellular profiles existed in deep than in superficial layers. In conclusion, VIP interneurons innervate non-GABAergic excitatory neurons and interneurons at their subcellular domains with layer-dependent specificity. This suggests a diverse output of VIP interneurons, which predicts multiple functionality in cortical circuitry beyond disinhibition.
Issue Date
2017
Journal
Cerebral Cortex 
eISSN
1460-2199

Export Metadata

Reference

Citations

31 citations in SCOPUS
1 citations in WoS
Usage 2 Download(s)

Social Media