Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots.

2018 | journal article. A publication with affiliation to the University of Göttingen.

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​Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots.​
Ramos-Gomes, F.; Bode, J.; Sukhanova, A.; Bozrova, S. V.; Saccomano, M.; Mitkovski, M. & Krueger, J. E. et al.​ (2018) 
Scientific Reports8(1) art. 4595​.​ DOI: 

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Ramos-Gomes, Fernanda; Bode, Julia; Sukhanova, Alyona; Bozrova, Svetlana V.; Saccomano, Mara; Mitkovski, Miso; Krueger, Julia Eva; Wege, Anja K.; Stuehmer, Walter; Samokhvalov, Pavel S.; Baty, Daniel; Chames, Patrick; Nabiev, Igor; Alves, Frauke 
Early detection of malignant tumours and, especially, micrometastases and disseminated tumour cells is still a challenge. In order to implement highly sensitive diagnostic tools we demonstrate the use of nanoprobes engineered from nanobodies (single-domain antibodies, sdAbs) and fluorescent quantum dots (QDs) for single- and two-photon detection and imaging of human micrometastases and disseminated tumour cells in ex vivo biological samples of breast and pancreatic metastatic tumour mouse models expressing human epidermal growth factor receptor 2 (HER2) or carcinoembryonic antigen (CEA). By staining thin (5-10 µm) paraffin and thick (50 µm) agarose tissue sections, we detected HER2- and CEA-positive human tumour cells infiltrating the surrounding tissues or metastasizing to different organs, including the brain, testis, lung, liver, and lymph nodes. Compared to conventional fluorescently labelled antibodies the sdAb-HER2-QD and sdAb-CEA-QD nanoprobes are superior in detecting micrometastases in tissue sections by lower photobleaching and higher brightness of fluorescence signals ensuring much better discrimination of positive signals versus background. Very high two-photon absorption cross-sections of QDs and small size of the nanoprobes ensure efficient imaging of thick tissue sections unattainable with conventional fluorescent probes. The nanobody-QD probes will help to improve early cancer diagnosis and prognosis of progression by assessing metastasis.
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Scientific Reports 
Max-Planck-Institut für Experimentelle Medizin ; Klinik für Hämatologie und Medizinische Onkologie 



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