Activin A increases phagocytosis of Escherichia coli K1 by primary murine microglial cells activated by toll-like receptor agonists

2018 | journal article. A publication with affiliation to the University of Göttingen.

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​Diesselberg, C., Ribes, S., Seele, J., Kaufmann, A., Redlich, S., Bunkowski, S., Hanisch, Uwe-Karsten ... Schütze, S. (2018). ​Activin A increases phagocytosis of Escherichia coli K1 by primary murine microglial cells activated by toll-like receptor agonists. Journal of Neuroinflammation15(1), Article 175​. ​doi: https://doi.org/10.1186/s12974-018-1209-2 

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Authors
Diesselberg, Catharina; Ribes, Sandra; Seele, Jana; Kaufmann, Annika; Redlich, Sandra; Bunkowski, Stephanie; Hanisch, Uwe-Karsten; Michel, Uwe; Nau, Roland; Schütze, Sandra
Abstract
BACKGROUND: Bacterial meningitis is associated with high mortality and long-term neurological sequelae. Increasing the phagocytic activity of microglia could improve the resistance of the CNS against infections. We studied the influence of activin A, a member of the TGF-β family with known immunoregulatory and neuroprotective effects, on the functions of microglial cells in vitro. METHODS: Primary murine microglial cells were treated with activin A (0.13 ng/ml-13 μg/ml) alone or in combination with agonists of TLR2, 4, and 9. Phagocytosis of Escherichia coli K1 as well as release of TNF-α, IL-6, CXCL1, and NO was assessed. RESULTS: Activin A dose-dependently enhanced the phagocytosis of Escherichia coli K1 by microglial cells activated by agonists of TLR2, 4, and 9 without further increasing NO and proinflammatory cytokine release. Cell viability of microglial cells was not affected by activin A. CONCLUSIONS: Priming of microglial cells with activin A could increase the elimination of bacteria in bacterial CNS infections. This preventive strategy could improve the resistance of the brain to infections, particularly in elderly and immunocompromised patients.
Issue Date
2018
Journal
Journal of Neuroinflammation 
Organization
Institut für Neuropathologie ; Klinik für Neurologie 
Language
English

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