Chromatin swelling drives neutrophil extracellular trap release

2018 | journal article. A publication of Göttingen

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​Chromatin swelling drives neutrophil extracellular trap release​
Neubert, E.; Meyer, D. ; Rocca, F.; Günay, G.; Kwaczala-Tessmann, A.; Grandke, J. & Senger-Sander, S. et al.​ (2018) 
Nature Communications9(1) art. 3767​.​ DOI: 

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Neubert, Elsa; Meyer, Daniel ; Rocca, Francesco; Günay, Gökhan; Kwaczala-Tessmann, Anja; Grandke, Julia; Senger-Sander, Susanne; Geisler, Claudia; Egner, Alexander ; Schön, Michael P.; Erpenbeck, Luise; Kruss, Sebastian
Neutrophilic granulocytes are able to release their own DNA as neutrophil extracellular traps (NETs) to capture and eliminate pathogens. DNA expulsion (NETosis) has also been documented for other cells and organisms, thus highlighting the evolutionary conservation of this process. Moreover, dysregulated NETosis has been implicated in many diseases, including cancer and inflammatory disorders. During NETosis, neutrophils undergo dynamic and dramatic alterations of their cellular as well as sub-cellular morphology whose biophysical basis is poorly understood. Here we investigate NETosis in real-time on the single-cell level using fluorescence and atomic force microscopy. Our results show that NETosis is highly organized into three distinct phases with a clear point of no return defined by chromatin status. Entropic chromatin swelling is the major physical driving force that causes cell morphology changes and the rupture of both nuclear envelope and plasma membrane. Through its material properties, chromatin thus directly orchestrates this complex biological process.
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Nature Communications 
Klinik für Dermatologie, Venerologie und Allergologie ; Institut für Physikalische Chemie ; DFG Forschungszentrum Molekularphysiologie des Gehirns und Exzellenzcluster Mikroskopie im Nanometerbereich ; Institut für Nanophotonik Göttingen e.V. 



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