DNA barcoding of flowering plants in Sumatra, Indonesia

2019 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​DNA barcoding of flowering plants in Sumatra, Indonesia​
Amandita, F. Y.; Rembold, K.; Vornam, B.; Rahayu, S.; Siregar, I. Z.; Kreft, H. & Finkeldey, R.​ (2019) 
Ecology and Evolution9(4) pp. 1858​-1868​.​ DOI: https://doi.org/10.1002/ece3.4875 

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Authors
Amandita, Fitri Y.; Rembold, Katja; Vornam, Barbara; Rahayu, Sri; Siregar, Iskandar Z.; Kreft, Holger; Finkeldey, Reiner
Abstract
The rapid conversion of Southeast Asian lowland rainforests into monocultures calls for the development of rapid methods for species identification to support ecological research and sustainable land-use management. Here, we investigated the utilization of DNA barcodes for identifying flowering plants from Sumatra, Indonesia. A total of 1,207 matK barcodes (441 species) and 2,376 rbcL barcodes (750 species) were successfully generated. The barcode effectiveness is assessed using four approaches: (a) comparison between morphological and molecular identification results, (b) best-close match analysis with TaxonDNA, (c) barcoding gap analysis, and (d) formation of monophyletic groups. Results show that rbcL has a much higher level of sequence recoverability than matK (95% and 66%). The comparison between morphological and molecular identifications revealed that matK and rbcL worked best assigning a plant specimen to the genus level. Estimates of identification success using best-close match analysis showed that >70% of the investigated species were correctly identified when using single barcode. The use of two-loci barcodes was able to increase the identification success up to 80%. The barcoding gap analysis revealed that neither matK nor rbcL succeeded to create a clear gap between the intraspecific and interspecific divergences. However, these two barcodes were able to discriminate at least 70% of the species from each other. Fifteen genera and twenty-one species were found to be nonmonophyletic with both markers. The two-loci barcodes were sufficient to reconstruct evolutionary relationships among the plant taxa in the study area that are congruent with the broadly accepted APG III phylogeny.
Issue Date
2019
Journal
Ecology and Evolution 
Project
SFB 990: Ökologische und sozioökonomische Funktionen tropischer Tieflandregenwald-Transformationssysteme (Sumatra, Indonesien) 
SFB 990 | B | B06: Taxonomische, funktionelle, phylogenetische und biogeographische Diversität vaskulärer Pflanzen in Regenwald-Transformationssystemen auf Sumatra (Indonesien) 
SFB 990 | Z | Z02: Central Scientific Support Unit 
Organization
Fakultät für Forstwissenschaften und Waldökologie ; Burckhardt-Institut ; Abteilung Biodiversität, Makroökologie und Biogeographie 
ISSN
2045-7758
Language
English
Subject(s)
sfb990_journalarticles

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