TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network

2018 | journal article. A publication with affiliation to the University of Göttingen.

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​TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network​
Renna, L.; Stefano, G.; Slabaugh, E.; Wormsbaecher, C.; Sulpizio, A.; Zienkiewicz, K. & Brandizzi, F.​ (2018) 
Nature Communications9(1) art. 5313​.​ DOI: https://doi.org/10.1038/s41467-018-07662-4 

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Authors
Renna, Luciana; Stefano, Giovanni; Slabaugh, Erin; Wormsbaecher, Clarissa; Sulpizio, Alan; Zienkiewicz, Krzysztof; Brandizzi, Federica
Abstract
Defining convergent and divergent mechanisms underlying the biogenesis and function of endomembrane organelles is fundamentally important in cell biology. In all eukaryotes, the Trans-Golgi Network (TGN) is the hub where the exocytic and endocytic pathways converge. To gain knowledge in the mechanisms underlying TGN biogenesis and function, we characterized TGNap1, a protein encoded by a plant gene of unknown function conserved with metazoans. We demonstrate that TGNap1 is a TGN protein required for the homeostasis of biosynthetic and endocytic traffic pathways. We also show that TGNap1 binds Rab6, YIP4 and microtubules. Finally, we establish that TGNap1 contributes to microtubule-dependent biogenesis, tracking and function of a TGN subset, likely through interaction with Rab6 and YIP4. Our results identify an important trafficking determinant at the plant TGN and reveal an unexpected reliance of post-Golgi traffic homeostasis and organelle biogenesis on microtubules in plants.
Issue Date
2018
Journal
Nature Communications 
Project
info:eu-repo/grantAgreement/EC/FP7/627266/EU//ALGAEOILSYNTH
Organization
Albrecht-von-Haller-Institut für Pflanzenwissenschaften ; Abteilung Biochemie der Pflanze 
ISSN
2041-1723
Language
English

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