Massive up-regulation of LBD transcription factors and EXPANSINs highlights the regulatory programs of rhizomania disease

2018 | journal article. A publication with affiliation to the University of Göttingen.

Jump to:Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​Fernando Gil, Jose, Sebastian Liebe, Heike Thiel, Britt-Louise Lennefors, Thomas Kraft, David Gilmer, Edgar Maiss, Mark Varrelmann, and Eugene I. Savenkov. "Massive up-regulation of LBD transcription factors and EXPANSINs highlights the regulatory programs of rhizomania disease​." ​Molecular Plant Pathology ​19, no. 10 (2018): ​2333​-2348​. ​https://doi.org/10.1111/mpp.12702.

Documents & Media

License

Published Version

Special user license Goescholar License

Details

Authors
Fernando Gil, Jose; Liebe, Sebastian; Thiel, Heike; Lennefors, Britt-Louise; Kraft, Thomas; Gilmer, David; Maiss, Edgar; Varrelmann, Mark; Savenkov, Eugene I.
Abstract
Rhizomania of sugar beet, caused by Beet necrotic yellow vein virus (BNYVV), is characterized by excessive lateral root (LR) formation leading to dramatic reduction of taproot weight and massive yield losses. LR formation represents a developmental process tightly controlled by auxin signaling through AUX/IAA-ARF responsive module and LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcriptional network. Several LBD transcription factors play central roles in auxin-regulated LR development and act upstream of EXPANSINS (EXPs), cell wall (CW)-loosening proteins involved in plant development via disruption of the extracellular matrix for CW relaxation and expansion. Here, we present evidence that BNYVV hijacks these auxin-regulated pathways resulting in formation LR and root hairs (RH). We identified an AUX/IAA protein (BvAUX28) as interacting with P25, a viral virulence factor. Mutational analysis indicated that P25 interacts with domains I and II of BvAUX28. Subcellular localization of co-expressed P25 and BvAUX28 showed that P25 inhibits BvAUX28 nuclear localization. Moreover, root-specific LBDs and EXPs were greatly upregulated during rhizomania development. Based on these data, we present a model in which BNYVV P25 protein mimics action of auxin by removing BvAUX28 transcriptional repressor, leading to activation of LBDs and EXPs. Thus, the evidence highlights two pathways operating in parallel and leading to uncontrolled formation of LRs and RHs, the main manifestation of the rhizomania syndrome.
Issue Date
2018
Journal
Molecular Plant Pathology 
Organization
Institut für Zuckerrübenforschung ; Fakultät für Agrarwissenschaften ; Department für Nutzpflanzenwissenschaften ; Abteilung Molekulare Phytopathologie und Mykotoxinforschung 
ISSN
1364-3703
Language
English

Reference

Citations


Social Media