Proteome profiling in murine models of multiple sclerosis: identification of stage specific markers and culprits for tissue damage.

2009-10-28 | Zeitschriftenartikel. Eine Publikation mit Affiliation zur Georg-August-Universität Göttingen.

Spring zu: Zitieren & Links | Dokumente & Medien | Details | Versionsgeschichte

Zitiervorschlag

​Proteome profiling in murine models of multiple sclerosis: identification of stage specific markers and culprits for tissue damage.​
Linker, R. A.; Brechlin, P.; Jesse, S.; Steinacker, P.; Lee, D. H.; Asif, A. R. & Jahn, O.  u.a.​ (2009) 
PLoS One4(10) art. e7624​.​ DOI: https://doi.org/10.1371/journal.pone.0007624 

Dokumente & Medien

Linker.pdf378.46 kBAdobe PDF

Lizenz

Published Version

Attribution 2.5 CC BY 2.5

Details

Autor(en)
Linker, Ralf A.; Brechlin, Peter; Jesse, Sarah; Steinacker, Petra; Lee, D. H.; Asif, Abdul R.; Jahn, Olaf ; Tumani, Hayrettin; Gold, Ralf; Otto, Markus
Zusammenfassung
The identification of new biomarkers is of high interest for the prediction of the disease course and also for the identification of pathomechanisms in multiple sclerosis (MS). To specify markers of the chronic disease phase, we performed proteome profiling during the later phase of myelin oligodendrocyte glycoprotein induced experimental autoimmune encephalomyelitis (MOG-EAE, day 35 after immunization) as a model disease mimicking many aspects of secondary progressive MS. In comparison to healthy controls, high resolution 2 dimensional gel electrophoresis revealed a number of regulated proteins, among them glial fibrilary acidic protein (GFAP). Phase specific up-regulation of GFAP in chronic EAE was confirmed by western blotting and immunohistochemistry. Protein levels of GFAP were also increased in the cerebrospinal fluid of MS patients with specificity for the secondary progressive disease phase. In a next step, proteome profiling of an EAE model with enhanced degenerative mechanisms revealed regulation of alpha-internexin, syntaxin binding protein 1, annexin V and glutamate decarboxylase in the ciliary neurotrophic factor (CNTF) knockout mouse. The identification of these proteins implicate an increased apoptosis and enhanced axonal disintegration and correlate well the described pattern of tissue injury in CNTF -/- mice which involve oligodendrocyte (OL) apoptosis and axonal injury.In summary, our findings underscore the value of proteome analyses as screening method for stage specific biomarkers and for the identification of new culprits for tissue damage in chronic autoimmune demyelination.
Erscheinungsdatum
28-Oktober-2009
Zeitschrift
PLoS One 
ISSN
1932-6203
Umfang
9
Sprache
Englisch

Export Metadaten

Referenzen

Zitationen


Social Media