Direct characterization of cytoskeletal reorganization during blood platelet spreading

A publication (journal article; original work) of the University of Göttingen

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​Direct characterization of cytoskeletal reorganization during blood platelet spreading​
Paknikar, A. K.; Eltzner, B.   & Köster, S. ​ (2019) 
Progress in biophysics and molecular biology144 pp. 166​-176​.​

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Authors
Paknikar, Aishwarya K.
Eltzner, Benjamin 
Köster, Sarah 
Abstract
Blood platelets are the key cellular players in blood clotting and thus of great biomedical importance. While spreading at the site of injury, they reorganize their cytoskeleton within minutes and assume a flat appearance. As platelets possess no nucleus, many standard methods for visualizing cytoskeletal components by means of fluorescence tags fail. Here we employ silicon-rhodamine actin and tubulin probes for imaging these important proteins in a time-resolved manner. We find two distinct timescales for platelet spread area development and for cytoskeletal reorganization, indicating that although cell spreading is most likely associated with actin polymerization at the cell edges, distinct, stress-fiber-like actin structures within the cell, which may be involved in the generation of contractile forces, form on their own timescale. Following microtubule dynamics allows us to distinguish the role of myosin, microtubules and actin during early spreading.
Issue Date
2019
Type
Journal Article
Subtype
original_ja
Journal
Progress in biophysics and molecular biology 
Organization
Institut für Röntgenphysik 
Fakultät für Physik 
Working Group
RG Köster (Cellular Biophysics) 
ISSN
0079-6107
eISSN
1873-1732
Language
English
Subject(s)
cellular biophysics

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