MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex

2020-01-07 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex​
Wang, C.; Richter-Dennerlein, R. ; Pacheu-Grau, D.; Liu, F.; Zhu, Y.; Dennerlein, S. & Rehling, P. ​ (2020) 
EMBO Reports21(1) art. e48833​.​ DOI: 

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Wang, Cong; Richter-Dennerlein, Ricarda ; Pacheu-Grau, David; Liu, Fan; Zhu, Ying; Dennerlein, Sven; Rehling, Peter 
The mitochondrial genome encodes for thirteen core subunits of the oxidative phosphorylation system. These proteins assemble with imported proteins in a modular manner into stoichiometric enzyme complexes. Assembly factors assist in these biogenesis processes by providing co-factors or stabilizing transient assembly stages. However, how expression of the mitochondrial-encoded subunits is regulated to match the availability of nuclear-encoded subunits is still unresolved. Here, we address the function of MITRAC15/COA1, a protein that participates in complex I biogenesis and complex IV biogenesis. Our analyses of a MITRAC15 knockout mutant reveal that MITRAC15 is required for translation of the mitochondrial-encoded complex I subunit ND2. We find that MITRAC15 is a constituent of a ribosome-nascent chain complex during ND2 translation. Chemical crosslinking analyses demonstrate that binding of the ND2-specific assembly factor ACAD9 to the ND2 polypeptide occurs at the C-terminus and thus downstream of MITRAC15. Our analyses demonstrate that expression of the founder subunit ND2 of complex I undergoes regulation. Moreover, a ribosome-nascent chain complex with MITRAC15 is at the heart of this process.
Issue Date
EMBO Reports 
EXC 2067: Multiscale Bioimaging 
Working Group
RG Rehling (Mitochondrial Protein Biogenesis) 
External URL
1469-221X; 1469-3178



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