Nanoscale separation of molecular species based on their rotational mobility

2008 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Nanoscale separation of molecular species based on their rotational mobility​
Testa, I. ; Schönle, A. ; von Middendorff, C.; Geisler, C. ; Medda, R. ; Wurm, C. A.   & Stiel, A. C.  et al.​ (2008) 
Optics Express16(25) pp. 21093​-21104​.​ DOI: https://doi.org/10.1364/OE.16.021093 

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Authors
Testa, Ilaria ; Schönle, Andreas ; von Middendorff, Claas; Geisler, Claudia ; Medda, Rebecca ; Wurm, Christian A. ; Stiel, Andre C. ; Jakobs, Stefan ; Bossi, Mariano ; Eggeling, Christian ; Hell, Stefan W. ; Egner, Alexander 
Abstract
We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Subpopulations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells. (C) 2008 Optical Society of America
Issue Date
2008
Journal
Optics Express 
ISSN
1094-4087
Language
English

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