Mic60 exhibits a coordinated clustered distribution along and across yeast and mammalian mitochondria

2019 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Mic60 exhibits a coordinated clustered distribution along and across yeast and mammalian mitochondria​
Stoldt, S. ; Stephan, T. ; Jans, D. C. ; Brüser, C. ; Lange, F.; Keller-Findeisen, J.   & Riedel, D.  et al.​ (2019) 
Proceedings of the National Academy of Sciences116(20) pp. 9853​-9858​.​ DOI: https://doi.org/10.1073/pnas.1820364116 

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Stoldt, Stefan ; Stephan, Till ; Jans, Daniel C. ; Brüser, Christian ; Lange, Felix; Keller-Findeisen, Jan ; Riedel, Dietmar ; Hell, Stefan W. ; Jakobs, Stefan 
Mitochondria are tubular double-membrane organelles essential for eukaryotic life. They form extended networks and exhibit an intricate inner membrane architecture. The MICOS (mitochondrial contact site and cristae organizing system) complex, crucial for proper architecture of the mitochondrial inner membrane, is localized primarily at crista junctions. Harnessing superresolution fluorescence microscopy, we demonstrate that Mic60, a subunit of the MICOS complex, as well as several of its interaction partners are arranged into intricate patterns in human and yeast mitochondria, suggesting an ordered distribution of the crista junctions. We show that Mic60 forms clusters that are preferentially localized in the inner membrane at two opposing sides of the mitochondrial tubules so that they form extended opposing distribution bands. These Mic60 distribution bands can be twisted, resulting in a helical arrangement. Focused ion beam milling-scanning electron microscopy showed that in yeast the twisting of the opposing distribution bands is echoed by the folding of the inner membrane. We show that establishment of the Mic60 distribution bands is largely independent of the cristae morphology. We suggest that Mic60 is part of an extended multiprotein interaction network that scaffolds mitochondria.
Issue Date
Proceedings of the National Academy of Sciences 
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente 
SFB 1190 | P01: Untersuchung der Unterschiede in der Zusammensetzung, Funktion und Position von individuellen MICOS Komplexen in einzelnen Säugerzellen 
Working Group
RG Jakobs (Structure and Dynamics of Mitochondria) 
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