Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI
2018 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI
Weill, U.; Arakel, E. C. ; Goldmann, O.; Golan, M.; Chuartzman, S.; Munro, S. & Schwappach, B. et al. (2018)
Traffic, 19(5) pp. 370-379. DOI: https://doi.org/10.1111/tra.12560
Documents & Media
Details
- Authors
- Weill, Uri; Arakel, Eric C. ; Goldmann, Omer; Golan, Matan; Chuartzman, Silvia; Munro, Sean; Schwappach, Blanche ; Schuldiner, Maya
- Abstract
- A third of yeast genes encode for proteins that function in the endomembrane system. However, the precise localization for many of these proteins is still uncertain. Here, we visualized a collection of ~500 N-terminally, green fluorescent protein (GFP), tagged proteins of the yeast Saccharomyces cerevisiae. By co-localizing them with 7 known markers of endomembrane compartments we determined the localization for over 200 of them. Using this approach, we create a systematic database of the various secretory compartments and identify several new residents. Focusing in, we now suggest that Lam5 resides in contact sites between the endoplasmic reticulum and the late Golgi. Additionally, analysis of interactions between the COPI coat and co-localizing proteins from our screen identifies a subset of proteins that are COPI-cargo. In summary, our approach defines the protein roster within each compartment enabling characterization of the physical and functional organization of the endomembrane system and its components.
- Issue Date
- 2018
- Journal
- Traffic
- Project
- SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente
SFB 1190 | P04: Der GET-Rezeptor als ein Eingangstor zum ER und sein Zusammenspiel mit GET bodies
SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel
SFB 1190 | Z03: Synthetische genetische Analyse, automatisierte Mikroskopie und Bildanalyse - Working Group
- RG Schuldiner (Functional Genomics of Organelles)
RG Schwappach (Membrane Protein Biogenesis) - External URL
- https://sfb1190.med.uni-goettingen.de/production/literature/publications/24
- ISSN
- 1398-9219
- Language
- English