Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI

2018 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI​
Weill, U.; Arakel, E. C. ; Goldmann, O.; Golan, M.; Chuartzman, S.; Munro, S. & Schwappach, B.  et al.​ (2018) 
Traffic19(5) pp. 370​-379​.​ DOI: https://doi.org/10.1111/tra.12560 

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Authors
Weill, Uri; Arakel, Eric C. ; Goldmann, Omer; Golan, Matan; Chuartzman, Silvia; Munro, Sean; Schwappach, Blanche ; Schuldiner, Maya 
Abstract
A third of yeast genes encode for proteins that function in the endomembrane system. However, the precise localization for many of these proteins is still uncertain. Here, we visualized a collection of ~500 N-terminally, green fluorescent protein (GFP), tagged proteins of the yeast Saccharomyces cerevisiae. By co-localizing them with 7 known markers of endomembrane compartments we determined the localization for over 200 of them. Using this approach, we create a systematic database of the various secretory compartments and identify several new residents. Focusing in, we now suggest that Lam5 resides in contact sites between the endoplasmic reticulum and the late Golgi. Additionally, analysis of interactions between the COPI coat and co-localizing proteins from our screen identifies a subset of proteins that are COPI-cargo. In summary, our approach defines the protein roster within each compartment enabling characterization of the physical and functional organization of the endomembrane system and its components.
Issue Date
2018
Journal
Traffic 
Project
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente 
SFB 1190 | P04: Der GET-Rezeptor als ein Eingangstor zum ER und sein Zusammenspiel mit GET bodies 
SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel 
SFB 1190 | Z03: Synthetische genetische Analyse, automatisierte Mikroskopie und Bildanalyse 
Working Group
RG Schuldiner (Functional Genomics of Organelles) 
RG Schwappach (Membrane Protein Biogenesis) 
External URL
https://sfb1190.med.uni-goettingen.de/production/literature/publications/24
ISSN
1398-9219
Language
English

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