Monitoring mitochondrial translation in living cells

2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Monitoring mitochondrial translation in living cells​
Yousefi, R.; Fornasiero, E. F ; Cyganek, L. ; Montoya, J.; Jakobs, S. ; Rizzoli, S. O   & Rehling, P.  et al.​ (2021) 
EMBO reports22(4).​ DOI: https://doi.org/10.15252/embr.202051635 

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Authors
Yousefi, Roya; Fornasiero, Eugenio F ; Cyganek, Lukas ; Montoya, Julio; Jakobs, Stefan ; Rizzoli, Silvio O ; Rehling, Peter ; Pacheu‐Grau, David 
Abstract
Abstract Mitochondria possess a small genome that codes for core subunits of the oxidative phosphorylation system and whose expression is essential for energy production. Information on the regulation and spatial organization of mitochondrial gene expression in the cellular context has been difficult to obtain. Here we devise an imaging approach to analyze mitochondrial translation within the context of single cells, by following the incorporation of clickable non‐canonical amino acids. We apply this method to multiple cell types, including specialized cells such as cardiomyocytes and neurons, and monitor with spatial resolution mitochondrial translation in axons and dendrites. We also show that translation imaging allows to monitor mitochondrial protein expression in patient fibroblasts. Approaching mitochondrial translation with click chemistry opens new avenues to understand how mitochondrial biogenesis is integrated into the cellular context and can be used to assess mitochondrial gene expression in mitochondrial diseases.
Synopsis image This study monitors mitochondrial protein synthesis with spatial resolution in single cells of multiple cell types. Labelling of mitochondrial translation products allows to monitor translation with spatial resolution within single cells. Mitochondria show different levels of protein synthesis within a single cell. Protein synthesis occurs in mitochondria of the pre‐ and the postsynapse.
This study monitors mitochondrial protein synthesis with spatial resolution in single cells of multiple cell types. image
Issue Date
2021
Journal
EMBO reports 
Project
EXC 2067: Multiscale Bioimaging 
SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz 
SFB 1002 | S01: In vivo und in vitro Krankheitsmodelle 
SFB 1286: Quantitative Synaptologie 
SFB 1286 | A05: Mitochondriale Heterogenität in Synapsen 
SFB 1286 | A06: Mitochondrienfunktion und -umsatz in Synapsen 
SFB 1286 | Z03: Unkomplizierte multispektrale, superauflösende Bildgebung durch zehnfache Expansionsmikroskopie 
Working Group
RG Jakobs (Structure and Dynamics of Mitochondria) 
RG Rehling (Mitochondrial Protein Biogenesis) 
RG Rizzoli (Quantitative Synaptology in Space and Time) 
RG Cyganek (Stem Cell Unit) 
External URL
https://mbexc.uni-goettingen.de/literature/publications/224
https://sfb1002.med.uni-goettingen.de/production/literature/publications/388
https://sfb1286.uni-goettingen.de/literature/publications/107
ISSN
1469-221X
eISSN
1469-3178
Language
English
Sponsor
European Research Council (ERC) http://dx.doi.org/10.13039/501100000781
Sonderforschungsbereiche (SFB)
Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
Germany’s Excellence Strategy
German Federal Ministry of Education and Research (BMBF)/DZHK
Instituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587
Max Planck Institute for Biophysical Chemistry

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