Ultrastructural maturation of the endbulb of Held active zones comparing wild-type and otoferlin-deficient mice

2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.

Jump to:Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​Ultrastructural maturation of the endbulb of Held active zones comparing wild-type and otoferlin-deficient mice​
Hintze, A.; Gültas, M.; Semmelhack, E. A. & Wichmann, C. ​ (2021) 
iScience24(4) pp. 102282​.​ DOI: https://doi.org/10.1016/j.isci.2021.102282 

Documents & Media

License

Published Version

Attribution-NonCommercial-NoDerivs 4.0 CC BY-NC-ND 4.0

Details

Authors
Hintze, Anika; Gültas, Mehmet; Semmelhack, Esther A.; Wichmann, Carolin 
Abstract
Endbulbs of Held are located in the anteroventral cochlear nucleus and present the first central synapses of the auditory pathway. During development, endbulbs mature functionally to enable rapid and powerful synaptic transmission with high temporal precision. This process is accompanied by morphological changes of endbulb terminals. Loss of the hair cell-specific protein otoferlin (Otof) abolishes neurotransmission in the cochlea and results in the smaller endbulb of Held terminals. Thus, peripheral hearing impairment likely also leads to alterations in the morphological synaptic vesicle (SV) pool size at individual endbulb of Held active zones (AZs). Here, we investigated endbulb AZs in pre-hearing, young, and adult wild-type and Otof−/− mice. During maturation, SV numbers at endbulb AZs increased in wild-type mice but were found to be reduced in Otof−/− mice. The SV population at a distance of 0–15 nm was most strongly affected. Finally, overall SV diameters decreased in Otof−/− animals during maturation.
Issue Date
2021
Journal
iScience 
Project
SFB 1286: Quantitative Synaptologie 
SFB 1286 | A04: Aktivitätsabhängige morphologische Veränderungen am Endkolben von Held-Synapsen 
Working Group
RG Wichmann (Molecular Architecture of Synapses) 
External URL
https://sfb1286.uni-goettingen.de/literature/publications/112
ISSN
2589-0042
Language
English

Reference

Citations


Social Media