Three-dimensional imaging of xylem at cell wall level through near field nano holotomography

2021 | journal article. A publication with affiliation to the University of Göttingen.

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​Three-dimensional imaging of xylem at cell wall level through near field nano holotomography​
Koddenberg, T.; Greving, I.; Hagemann, J.; Flenner, S.; Krause, A.; Laipple, D. & Klein, K. C. et al.​ (2021) 
Scientific Reports11(1).​ DOI: 

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Koddenberg, Tim; Greving, Imke; Hagemann, Johannes; Flenner, Silja; Krause, Andreas; Laipple, Daniel; Klein, Kim C.; Schmitt, Uwe; Schuster, Max; Nopens, Martin
Abstract Detailed imaging of the three-dimensionally complex architecture of xylary plants is important for studying biological and mechanical functions of woody plants. Apart from common two-dimensional microscopy, X-ray micro-computed tomography has been established as a three-dimensional (3D) imaging method for studying the hydraulic function of wooden plants. However, this X-ray imaging method can barely reach the resolution needed to see the minute structures (e.g. pit membrane). To complement the xylem structure with 3D views at the nanoscale level, X-ray near-field nano-holotomography (NFH) was applied to analyze the wood species Pinus sylvestris and Fagus sylvatica . The demanded small specimens required focused ion beam (FIB) application. The FIB milling, however, influenced the image quality through gallium implantation on the cell-wall surfaces. The measurements indicated that NFH is appropriate for imaging wood at nanometric resolution. With a 26 nm voxel pitch, the structure of the cell-wall surface in Pinus sylvestris could be visualized in genuine detail. In wood of Fagus sylvatica , the structure of a pit pair, including the pit membrane, between two neighboring fibrous cells could be traced tomographically.
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Scientific Reports 
Fakultät für Forstwissenschaften und Waldökologie ; Burckhardt-Institut ; Abteilung Holzbiologie und Holzprodukte 



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