A fluorescent probe for STED microscopy to study NIP-specific B cells
2021 | journal article. A publication with affiliation to the University of Göttingen.
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Details
- Authors
- Glowacki, Selda Kabatas; Gomes de Castro, Maria Angela ; Yip, Ka Man; Asadpour, Ommolbanin; Münchhalfen, Matthias; Engels, Niklas; Opazo, Felipe
- Abstract
- Monovalent NIP probes for studying B cell antigen receptors in fluorescence-based techniques, including diffraction unlimited microscopy.
We have developed a series of monovalent fluorophore-conjugated affinity probes based on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model antigen to study B lymphocytes and the functional principles of B cell antigen receptors (BCRs). We successfully used them in flow-cytometry, confocal and super-resolution microscopy techniques.
Monovalent NIP probes for studying B cell antigen receptors in fluorescence-based techniques, including diffraction unlimited microscopy.
We have developed a series of monovalent fluorophore-conjugated affinity probes based on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model antigen to study B lymphocytes and the functional principles of B cell antigen receptors (BCRs). We successfully used them in flow-cytometry, confocal and super-resolution microscopy techniques. - Issue Date
- 2021
- Journal
- The Analyst
- ISSN
- 0003-2654
- eISSN
- 1364-5528
- Language
- English
