Structural basis of RNA processing by human mitochondrial RNase P

2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Bhatta, Arjun, et al. "Structural basis of RNA processing by human mitochondrial RNase P​." ​Nature Structural & Molecular Biology, vol. 28, no. 9, ​2021, pp. 713​-723​, ​doi: 10.1038/s41594-021-00637-y. 

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Authors
Bhatta, Arjun; Dienemann, Christian ; Cramer, Patrick ; Hillen, Hauke S. 
Abstract
Abstract Human mitochondrial transcripts contain messenger and ribosomal RNAs flanked by transfer RNAs (tRNAs), which are excised by mitochondrial RNase (mtRNase) P and Z to liberate all RNA species. In contrast to nuclear or bacterial RNase P, mtRNase P is not a ribozyme but comprises three protein subunits that carry out RNA cleavage and methylation by unknown mechanisms. Here, we present the cryo-EM structure of human mtRNase P bound to precursor tRNA, which reveals a unique mechanism of substrate recognition and processing. Subunits TRMT10C and SDR5C1 form a subcomplex that binds conserved mitochondrial tRNA elements, including the anticodon loop, and positions the tRNA for methylation. The endonuclease PRORP is recruited and activated through interactions with its PPR and nuclease domains to ensure precise pre-tRNA cleavage. The structure provides the molecular basis for the first step of RNA processing in human mitochondria.
Issue Date
2021
Journal
Nature Structural & Molecular Biology 
Project
EXC 2067: Multiscale Bioimaging 
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente 
FOR 2848: Architektur und Heterogenität der inneren mitochondrialen Membran auf der Nanoskala 
FOR 2848 | St01: Structure and distribution of ribosomes at the inner mitochondrial membrane 
Working Group
RG Cramer 
RG Hillen (Structure and Function of Molecular Machines) 
External URL
https://mbexc.uni-goettingen.de/literature/publications/337
https://sfb1190.med.uni-goettingen.de/production/literature/publications/154
https://for2848.gwdguser.de/literature/publications/9
ISSN
1545-9993
eISSN
1545-9985
Language
English

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