The mammalian rod synaptic ribbon is essential for Cav channel facilitation and ultrafast synaptic vesicle fusion

2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​The mammalian rod synaptic ribbon is essential for Cav channel facilitation and ultrafast synaptic vesicle fusion​
Grabner, C. P.   & Moser, T. ​ (2021) 
eLife10.​ DOI: https://doi.org/10.7554/eLife.63844 

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Authors
Grabner, Chad Paul ; Moser, Tobias 
Abstract
Rod photoreceptors (PRs) use ribbon synapses to transmit visual information. To signal ‘no light detected’ they release glutamate continually to activate post-synaptic receptors. When light is detected glutamate release pauses. How a rod’s individual ribbon enables this process was studied here by recording evoked changes in whole-cell membrane capacitance from wild-type and ribbonless ( Ribeye -ko) mice. Wild-type rods filled with high (10 mM) or low (0.5 mM) concentrations of the Ca 2+ -buffer EGTA created a readily releasable pool (RRP) of 87 synaptic vesicles (SVs) that emptied as a single kinetic phase with a τ<0.4 ms. The lower concentration of EGTA accelerated Ca v channel opening and facilitated release kinetics. In contrast, ribbonless rods created a much smaller RRP of 22 SVs, and they lacked Ca v channel facilitation; however, Ca 2+ channel-release coupling remained tight. These release deficits caused a sharp attenuation of rod-driven scotopic light responses. We conclude that the synaptic ribbon facilitates Ca 2+ -influx and establishes a large RRP of SVs.
Issue Date
2021
Journal
eLife 
Project
EXC 2067: Multiscale Bioimaging 
SFB 1286: Quantitative Synaptologie 
SFB 1286 | B05: Quantitative molekulare Physiologie aktiver Zonen in Calyx-Synapsen 
Working Group
RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding) 
External URL
https://mbexc.uni-goettingen.de/literature/publications/386
https://sfb1286.uni-goettingen.de/literature/publications/138
eISSN
2050-084X
Language
English

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