Mechanical stress effects on transcriptional regulation of genes encoding microtubule- and actin-associated proteins

2022 | journal article; research paper. A publication with affiliation to the University of Göttingen.

Jump to:Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​Shevchenko, G. V. & Krutovsky, K. V. (2022). ​Mechanical stress effects on transcriptional regulation of genes encoding microtubule- and actin-associated proteins. Physiology and Molecular Biology of Plants, . ​doi: https://doi.org/10.1007/s12298-021-01123-x 

Documents & Media

s12298-021-01123-x.pdf1.21 MBAdobe PDF

License

Published Version

Attribution 4.0 CC BY 4.0

Details

Authors
Shevchenko, Galina V.; Krutovsky, Konstantin V. 
Abstract
Abstract Plant cytoskeleton regulation has been studied using a new approach based on both (1) pharmacological analysis of tubulin and actin inhibitors and (2) mechanical stimulation achieved by using a slow-rotating (2 rpm) clinostat in combination with transcriptional analysis of genes encoding TUA6, ACT2, MAP65-1, CLASP, PLDδ, FH4 and FH1 proteins in Arabidopsis thaliana seedling roots. The obtained data suggest feedback between the organization of microtubule (MT) and actin filament (AF) networks and the expression of the ACT2, TUA6, MAP65-1, CLASP and FH1/FH4 genes. Different regulation of feedback between MT/AF organization and TUA6, ACT2, MAP65-1, CLASP, FH4 and FH1 gene expression was noted during slow clinorotation, possibly due to altered mechanical impact on the cortical cytoskeleton. For the first time, the expression of the tubulin-associated gene MAP65-1 was shown to be dependent upon the organization of AFs. TUA6, MAP65-1, CLASP, FH1 and FH4 likely participate in mechanical signal transduction. Our work demonstrated that slow clinorotation is able to cause mechanical stress.
Issue Date
2022
Journal
Physiology and Molecular Biology of Plants 
Organization
Fakultät für Forstwissenschaften und Waldökologie ; Büsgen-Institut ; Abteilung Forstgenetik und Forstpflanzenzüchtung ; Zentrum für Integrierte Züchtungsforschung 
ISSN
0971-5894
eISSN
0974-0430
Language
English

Reference

Citations


Social Media