Dual-Label STED Nanoscopy of Living Cells Using Photochromism
2011 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Willig, Katrin I. ; Stiel, Andre C. ; Brakemann, Tanja ; Jakobs, Stefan ; Hell, Stefan W.
- Abstract
- We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.
- Issue Date
- 2011
- Journal
- Nano Letters
- ISSN
- 1530-6984
- Language
- English