Dual-Label STED Nanoscopy of Living Cells Using Photochromism

2011 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Dual-Label STED Nanoscopy of Living Cells Using Photochromism​
Willig, K. I. ; Stiel, A. C. ; Brakemann, T. ; Jakobs, S.   & Hell, S. W. ​ (2011) 
Nano Letters11(9) pp. 3970​-3973​.​ DOI: https://doi.org/10.1021/nl202290w 

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Authors
Willig, Katrin I. ; Stiel, Andre C. ; Brakemann, Tanja ; Jakobs, Stefan ; Hell, Stefan W. 
Abstract
We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.
Issue Date
2011
Journal
Nano Letters 
ISSN
1530-6984
Language
English

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