Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
2017-07-01 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
Krenkel, M.; Toepperwien, M.; Alves, F. & Salditt, T. (2017)
Acta Crystallographica Section A Foundations and Advances, 73(4) pp. 282-292. DOI: https://doi.org/10.1107/S2053273317007902 10.1107/s2053273317007902
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Details
- Authors
- Krenkel, Martin; Toepperwien, Mareike; Alves, Frauke ; Salditt, Tim
- Abstract
- X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data.
X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data. - Issue Date
- 1-July-2017
- Journal
- Acta Crystallographica Section A Foundations and Advances
- Organization
- Institut für Röntgenphysik
- Working Group
- RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)
- ISSN
- 2053-2733
- eISSN
- 2053-2733
- Language
- English
- Subject(s)
- x-ray imaging; biomedical tomography