Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer
2022 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer
Thiele, J. C. ; Jungblut, M.; Helmerich, D. A.; Tsukanov, R.; Chizhik, A.; Chizhik, A. I. & Schnermann, M. J. et al. (2022)
Science Advances, 8(23). DOI: https://doi.org/10.1126/sciadv.abo2506
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Details
- Authors
- Thiele, Jan Christoph ; Jungblut, Marvin; Helmerich, Dominic A.; Tsukanov, Roman; Chizhik, Anna; Chizhik, Alexey I. ; Schnermann, Martin J.; Sauer, Markus; Nevskyi, Oleksii; Enderlein, Jörg
- Abstract
- Over the past two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution, which is by a factor of 2 to 3 worse. One recently developed method to close this gap is metal-induced energy transfer (MIET) imaging, which achieves an axial resolution down to nanometers. It exploits the distance-dependent quenching of fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we combine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule localization microscopy, in particular with direct stochastic optical reconstruction microscopy (dSTORM). This combination allows us to achieve isotropic three-dimensional super-resolution imaging of subcellular structures. Moreover, we used spectral demixing for implementing dual-color MIET-dSTORM that allows us to image and colocalize, in three dimensions, two different cellular structures simultaneously.
- Issue Date
- 2022
- Journal
- Science Advances
- Project
- EXC 2067: Multiscale Bioimaging
- Working Group
- RG Enderlein
- Language
- English
- Sponsor
- Open-Access-Publikationsfonds 2022