Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability
2006 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Bossi, Mariano L. ; Fölling, Jonas ; Dyba, Marcus ; Westphal, Volker ; Hell, Stefan
- Abstract
- We demonstrate the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by photoswitching ensembles of optically bistable organic molecular markers from a non-fluorescent to a fluorescent state and back. The photoswitching is accomplished by an isomerization reaction of a photochromic compound serving as a reversible energy acceptor of a fluorescent compound. The surpassing of the diffraction barrier with power levels of only a few hundred W cm(-2) of continuous wave irradiation is evidenced both in the effective point spread function and in the fluorescence images of test samples.
- Issue Date
- 2006
- Journal
- New Journal of Physics
- ISSN
- 1367-2630
- Language
- English