Genome-wide SWAp-Tag yeast libraries for proteome exploration
2018 | journal article; research paper
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Cite this publication
Genome-wide SWAp-Tag yeast libraries for proteome exploration
Weill, U.; Yofe, I.; Sass, E.; Stynen, B.; Davidi, D.; Natarajan, J. & Ben-Menachem, R. et al. (2018)
Nature Methods, 15(8) pp. 617-622. DOI: https://doi.org/10.1038/s41592-018-0044-9
Documents & Media
Details
- Authors
- Weill, Uri; Yofe, Ido; Sass, Ehud; Stynen, Bram; Davidi, Dan; Natarajan, Janani; Ben-Menachem, Reut; Avihou, Zohar; Goldman, Omer; Harpaz, Nofar; Chuartzman, Silvia; Kniazev, Kiril; Knoblach, Barbara; Laborenz, Janina; Boos, Felix; Kowarzyk, Jacqueline; Ben-Dor, Shifra; Zalckvar, Einat; Herrmann, Johannes M.; Rachubinski, Richard A.; Pines, Ophry; Rapaport, Doron; Michnick, Stephen W.; Levy, Emmanuel D.; Schuldiner, Maya
- Abstract
- Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.
- Issue Date
- 2018
- Journal
- Nature Methods
- Project
- SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente
SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel - Working Group
- RG Schuldiner (Functional Genomics of Organelles)
- ISSN
- 1548-7091
- eISSN
- 1548-7105
- Language
- English
