Rapid Ca2+ channel accumulation contributes to cAMP-mediated increase in transmission at hippocampal mossy fiber synapses
2021 | journal article; research paper
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Rapid Ca2+ channel accumulation contributes to cAMP-mediated increase in transmission at hippocampal mossy fiber synapses
Fukaya, R.; Maglione, M.; Sigrist, S. J. & Sakaba, T. (2021)
Proceedings of the National Academy of Sciences of the United States of America, 118(9). DOI: https://doi.org/10.1073/pnas.2016754118
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Details
- Authors
- Fukaya, Ryota; Maglione, Marta; Sigrist, Stephan J. ; Sakaba, Takeshi
- Abstract
- The cyclic adenosine monophosphate (cAMP)-dependent potentiation of neurotransmitter release is important for higher brain functions such as learning and memory. To reveal the underlying mechanisms, we applied paired pre- and postsynaptic recordings from hippocampal mossy fiber-CA3 synapses. Ca2+ uncaging experiments did not reveal changes in the intracellular Ca2+ sensitivity for transmitter release by cAMP, but suggested an increase in the local Ca2+ concentration at the release site, which was much lower than that of other synapses before potentiation. Total internal reflection fluorescence (TIRF) microscopy indicated a clear increase in the local Ca2+ concentration at the release site within 5 to 10 min, suggesting that the increase in local Ca2+ is explained by the simple mechanism of rapid Ca2+ channel accumulation. Consistently, two-dimensional time-gated stimulated emission depletion microscopy (gSTED) microscopy showed an increase in the P/Q-type Ca2+ channel cluster size near the release sites. Taken together, this study suggests a potential mechanism for the cAMP-dependent increase in transmission at hippocampal mossy fiber-CA3 synapses, namely an accumulation of active zone Ca2+ channels.
- Issue Date
- 2021
- Journal
- Proceedings of the National Academy of Sciences of the United States of America
- Project
- FOR 2705: Dissection of a Brain Circuit: Structure, Plasticity and Behavioral Function of the Drosophila Mushroom Body
FOR 2705 | TP 5: Postsynaptic receptor plasticity and transsynaptic communication in storage of memory components in the mushroom bodies - Working Group
- RG Sigrist (Genetics)
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Language
- English