N-Cyanorhodamines: cell-permeant, photostable and bathochromically shifted analogues of fluoresceins
2022 | journal article. A publication with affiliation to the University of Göttingen.
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- Authors
- Heynck, Lukas; Matthias, Jessica; Bossi, Mariano Luis ; Butkevich, Alexey N. ; Hell, Stefan W.
- Abstract
- Fluorescein and its analogues have found only limited use in biological imaging because of the poor photostability and cell membrane impermeability of their O-unprotected forms. Herein, we report rationally designed N-cyanorhodamines as orange- to red-emitting, photostable and cell-permeant fluorescent labels negatively charged at physiological pH values and thus devoid of off-targeting artifacts often observed for cationic fluorophores. In combination with well-established fluorescent labels, self-labelling protein (HaloTag, SNAP-tag) ligands derived from N-cyanorhodamines permit up to four-colour confocal and super-resolution STED imaging in living cells.
- Issue Date
- 2022
- Journal
- Chemical Science
- Organization
- Max-Planck-Institut für Multidisziplinäre Naturwissenschaften
- Working Group
- RG Hell
- ISSN
- 2041-6520
- eISSN
- 2041-6539
- Language
- English
- Sponsor
- Bundesministerium für Bildung und Forschung https://doi.org/10.13039/501100002347