In vivo super-resolution of the brain – How to visualize the hidden nanoplasticity?
2022 | journal article. A publication with affiliation to the University of Göttingen.
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Details
- Authors
- Willig, Katrin I.
- Abstract
- Super-resolution fluorescence microscopy has entered most biological laboratories worldwide and its benefit is undisputable. Its application to brain imaging, for example in living mice, enables the study of sub-cellular structural plasticity and brain function directly in a living mammal. The demands of brain imaging on the different super-resolution microscopy techniques (STED, RESOLFT, SIM, ISM) and labeling strategies are discussed here as well as the challenges of the required cranial window preparation. Applications of super-resolution in the anesthetized mouse brain enlighten the stability and plasticity of synaptic nanostructures. These studies show the potential of in vivo super-resolution imaging and justify its application more widely in vivo to investigate the role of nanostructures in memory and learning.
- Issue Date
- 2022
- Journal
- iScience
- Organization
- Max-Planck-Institut für Multidisziplinäre Naturwissenschaften
- Working Group
- RG Willig (Optical Nanoscopy in Neuroscience)
- ISSN
- 2589-0042
- Language
- English