Ancient DNA analysis from epoxy resin Biodur®-embedded bones
2022 | journal article. A publication with affiliation to the University of Göttingen.
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Details
- Authors
- Flux, Anna Lena ; Schultz, Michael ; Hummel, Susanne
- Abstract
- For microscopic investigation, archaeological bone samples are often embedded in Biodur® epoxy resin. This study wants to test whether it is possible to extract DNA suitable for PCR amplification from this sample type. For eight individuals a set of samples – each consisting of a Biodur-embedded femur sample, a native femur sample and a control sample of different anatomical origin – were submitted to organic DNA extraction. The extraction success was tested by autosomal short tandem repeat amplification. Seven out of eight Biodur-embedded femur samples revealed successful amplification results. If Biodur-embedded bone material exists from earlier microscopic investigations, our results encourage the use of this sample type as a source for genetic research.
- Issue Date
- 2022
- Journal
- BioTechniques
- Organization
- Johann-Friedrich-Blumenbach-Institut für Zoologie und Anthropologie ; Universitätsmedizin Göttingen ; Institut für Anatomie und Embryologie ; Abteilung Historische Anthropologie und Humanökologie
- ISSN
- 0736-6205
- eISSN
- 1940-9818
- Language
- English