Impaired Mitogen-Driven Proliferation and Cytokine Transcription of Lymphocytes from Macaques Early After Simian Immunodeficiency Virus (SIV) Infection
1997 | journal article; research paper
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Impaired Mitogen-Driven Proliferation and Cytokine Transcription of Lymphocytes from Macaques Early After Simian Immunodeficiency Virus (SIV) Infection
Spring, M.; Bodemer, W. ; Stahl-Hennig, C. ; Nißlein, T.; Hunsmann, G. & Dittmer, U. (1997)
Viral Immunology, 10(2) pp. 65-72. DOI: https://doi.org/10.1089/vim.1997.10.65
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Details
- Authors
- Spring, Michael; Bodemer, Walter ; Stahl-Hennig, Christiane ; Nißlein, Thomas; Hunsmann, Gerhard; Dittmer, Ulf
- Abstract
- Altered cytokine transcription might play an important role in the pathogenesis of human immunodeficiency virus (HIV) infection in humans. The infection of rhesus macaques with simian immunodeficiency virus (SIV) provides a relevant animal model for HIV infection. Therefore, we evaluated the cyokine transcription of phytohemagglutinin (PHA)-stimulated lymphocytes in the early phase after infection of four rhesus macaques with pathogenic SIV-mac239. To determine transcription of interleukin (IL)-2, interferon (IFN)-γ, IL-4, IL-6, and IL-10 we established a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). After inoculation with SIV, all monkeys became productively infected and developed an acquired immunodeficiency syndrome (AIDS) like disease. Infection was associated with a proliferation dysfunction of monkey lymphocytes in response to PHA. In addition, a decreasing overall cytokine transcription could be observed during the course of SIV infection. These findings demonstrate that an impairment of the lymphocyte function is associated with a reduced cytokine transcription in the early phase of an immunodeficiency virus infection. The observed differences of cytokine expression might contribute to the impaired immune response of SIV-infected monkeys and HIV-infected humans.
- Issue Date
- 1997
- Journal
- Viral Immunology
- Organization
- Deutsches Primatenzentrum
- ISSN
- 0882-8245
- eISSN
- 1557-8976
- Language
- English