Nanoscale resolution in GFP-based microscopy

2006 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Nanoscale resolution in GFP-based microscopy​
Willig, K. I. ; Kellner, R. R. ; Medda, R. ; Hein, B. ; Jakobs, S.   & Hell, S. W. ​ (2006) 
Nature Methods, 3(9) pp. 721​-723​.​ DOI: https://doi.org/10.1038/NMETH922 

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Authors
Willig, Katrin I. ; Kellner, Robert R. ; Medda, Rebecca ; Hein, Birka ; Jakobs, Stefan ; Hell, Stefan W. 
Abstract
We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The similar to 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.
Issue Date
2006
Journal
Nature Methods 
ISSN
1548-7091

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