Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
2014-09 | journal article
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Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
Arnlund, D.; Johansson, L. C.; Wickstrand, C.; Barty, A.; Williams, G. J.; Malmerberg, E. & Davidsson, J. et al. (2014)
Nature Methods, 11(9) pp. 923-926. DOI: https://doi.org/10.1038/nmeth.3067
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Details
- Authors
- Arnlund, David; Johansson, Linda C.; Wickstrand, Cecilia; Barty, Anton; Williams, Garth J.; Malmerberg, Erik; Davidsson, Jan; Milathianaki, Despina; DePonte, Daniel P.; Shoeman, Robert L.; Wang, Dingjie; James, Daniel; Katona, Gergely; Westenhoff, Sebastian; White, Thomas A.; Aquila, Andrew; Bari, Sadia; Berntsen, Peter; Bogan, Mike; van Driel, Tim Brandt; Doak, R. Bruce; Kjær, Kasper Skov; Frank, Matthias; Fromme, Raimund; Grotjohann, Ingo; Henning, Robert; Hunter, Mark S.; Kirian, Richard A.; Kosheleva, Irina; Kupitz, Christopher; Liang, Mengning; Martin, Andrew V.; Nielsen, Martin Meedom; Messerschmidt, Marc; Seibert, M. Marvin; Sjöhamn, Jennie; Stellato, Francesco; Weierstall, Uwe; Zatsepin, Nadia A.; Spence, John C. H.; Fromme, Petra; Schlichting, Ilme; Boutet, Sébastien; Groenhof, Gerrit; Chapman, Henry N.; Neutze, Richard
- Abstract
- We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.
- Issue Date
- September-2014
- Journal
- Nature Methods
- Working Group
- RG Techert (Structural Dynamics in Chemical Systems)
- ISSN
- 1548-7091
- eISSN
- 1548-7105
- Language
- English
