Optogenetics and electron tomography for structure-function analysis of cochlear ribbon synapses
2022 | journal article. A publication with affiliation to the University of Göttingen.
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Optogenetics and electron tomography for structure-function analysis of cochlear ribbon synapses
Chakrabarti, R.; Jaime Tobón, L. M.; Slitin, L.; Redondo-Canales, M.; Hoch, G.; Slashcheva, M. & Fritsch, E. et al. (2022)
eLife, 11 art. e79494. DOI: https://doi.org/10.7554/eLife.79494
Documents & Media
Details
- Authors
- Chakrabarti, Rituparna; Jaime Tobón, Lina María; Slitin, Loujin; Redondo-Canales, Magdalena; Hoch, Gerhard; Slashcheva, Marina; Fritsch, Elisabeth; Bodensiek, Kai; Özçete, Özge Demet; Gültas, Mehmet; Wichmann, Carolin
- Abstract
- Ribbon synapses of cochlear inner hair cells (IHCs) are specialized to indefatigably transmit sound information at high rates. To understand the underlying mechanisms, structure-function analysis of the active zone (AZ) of these synapses is essential. Previous electron microscopy studies of synaptic vesicle (SV) dynamics at the IHC AZ used potassium stimulation, which limited the temporal resolution to minutes. Here, we established optogenetic IHC stimulation followed by quick freezing within milliseconds and electron tomography to study the ultrastructure of functional synapse states with good temporal resolution in mice. We characterized optogenetic IHC stimulation by patch-clamp recordings from IHCs and postsynaptic boutons revealing robust IHC depolarization and transmitter release. Ultrastructurally, the number of docked SVs increased upon short (17-25 ms) and long (48-76 ms) light stimulation paradigms. We did not observe enlarged SVs or other morphological correlates of homotypic fusion events. Our results indicate a rapid recruitment of SVs to the docked state upon stimulation and suggest that univesicular release prevails as the quantal mechanism of exocytosis at IHC ribbon synapses.
- Issue Date
- 2022
- Journal
- eLife
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1286: Quantitative Synaptologie
SFB 1286 | A04: Aktivitätsabhängige morphologische Veränderungen am Endkolben von Held-Synapsen
SFB 1286 | B05: Quantitative molekulare Physiologie aktiver Zonen in Calyx-Synapsen
SFB 1286 | Z04: Quantitative Visualisierung und Analyse synaptischer Proteine mit Hilfe von Nanobodies - Working Group
- RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)
RG Pangršič Vilfan (Experimental Otology)
RG Wichmann (Molecular Architecture of Synapses) - eISSN
- 2050-084X
- Language
- English
- Sponsor
- Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
Multiscale Bioimaging is a Cluster of Excellence of the University of Göttingen, Germany
MPI-NAT
Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
Leibniz Program
Niedersächsisches Ministerium für Wissenschaft und Kultur http://dx.doi.org/10.13039/501100010570
Erasmus Mundus
Fondation Pour l'Audition http://dx.doi.org/10.13039/100019671