Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2
2006 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2
Andrews-Zwilling, Y. S.; Kawabe, H.; Reim, K.; Varoqueaux, F. & Brose, N. (2006)
Journal of biological chemistry, 281(28) pp. 19720-19731. DOI: https://doi.org/10.1074/jbc.M601421200
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- Authors
- Andrews-Zwilling, Yaisa S.; Kawabe, Hiroshi; Reim, Kerstin; Varoqueaux, Frederique; Brose, Nils
- Abstract
- Transmitter release at synapses between nerve cells is spatially restricted to active zones, where synaptic vesicle docking, priming, and Ca2+-dependent fusion take place in a temporally highly coordinated manner. Munc13s are essential for priming synaptic vesicles to a fusion competent state, and their specific active zone localization contributes to the active zone restriction of transmitter release and the speed of excitation-secretion coupling. However, the molecular mechanism of the active zone recruitment of Munc13s is not known. We show here that the active zone recruitment of Munc13 isoforms Munc13-1 and ubMunc13-2 is regulated by their binding to the Rab3A-interacting molecule RIM1 alpha, a key determinant of long term potentiation of synaptic transmission at mossy fiber synapses in the hippocampus. We identify a single point mutation in Munc13-1 and ubMunc13-2 (I121N) that, depending on the type of assay used, strongly perturbs or abolishes RIM1 alpha binding in vitro and in cultured fibroblasts, and we demonstrate that RIM1 alpha binding-deficient ubMunc13-2(I121) is not efficiently recruited to synapses. Moreover, the levels of Munc13-1 and ubMunc13-2 levels are decreased in RIM1 alpha-deficient brain, and Munc13-1 is not properly enriched at active zones of mossy fiber terminals of the mouse hippocampus if RIM1 alpha is absent. We conclude that one function of the Munc13/RIM1 alpha interaction is the active zone recruitment of Munc13-1 and ubMunc13-2.
- Issue Date
- 2006
- Publisher
- Amer Soc Biochemistry Molecular Biology Inc
- Journal
- Journal of biological chemistry
- ISSN
- 0021-9258
- eISSN
- 1083-351X