CD154 Costimulation Shifts the Local T-Cell Receptor Repertoire Not Only During Thymic Selection but Also During Peripheral T-Dependent Humoral Immune Responses
2018 | journal article. A publication with affiliation to the University of Göttingen.
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CD154 Costimulation Shifts the Local T-Cell Receptor Repertoire Not Only During Thymic Selection but Also During Peripheral T-Dependent Humoral Immune Responses
Fähnrich, A.; Klein, S.; Sergé, A.; Nyhoegen, C.; Kombrink, S.; Möller, S. & Keller, K. et al. (2018)
Frontiers in Immunology, 9 pp. 1019. DOI: https://doi.org/10.3389/fimmu.2018.01019
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Details
- Authors
- Fähnrich, Anke; Klein, Sebastian; Sergé, Arnauld; Nyhoegen, Christin; Kombrink, Sabrina; Möller, Steffen; Keller, Karsten; Westermann, Jürgen; Kalies, Kathrin
- Abstract
- CD154 is a transmembrane cytokine expressed transiently on activated CD4 T cells upon T-cell receptor (TCR) stimulation that interacts with CD40 on antigen-presenting cells. The signaling via CD154:CD40 is essential for B-cell maturation and germinal center formation and also for the final differentiation of CD4 T cells during T-dependent humoral immune responses. Recent data demonstrate that CD154 is critically involved in the selection of T-cell clones during the negative selection process in the thymus. Whether CD154 signaling influences the TCR repertoire during peripheral T-dependent humoral immune responses has not yet been elucidated. To find out, we used CD154-deficient mice and assessed the global TCRβ repertoire in T-cell zones (TCZ) of spleens by high-throughput sequencing after induction of a Th2 response to the multiepitopic antigen sheep red blood cells. Qualitative and quantitative comparison of the splenic TCZ-specific TCRβ repertoires revealed that CD154 deficiency shifts the distribution of Vβ-Jβ genes after antigen exposure. This data led to the conclusion that costimulation via CD154:CD40 during the interaction of T cells with CD40-matured B cells contributes to the recruitment of T-cell clones into the immune response and thereby shapes the peripheral TCR repertoire.
- Issue Date
- 2018
- Journal
- Frontiers in Immunology
- Organization
- Mathematisches Institut
- ISSN
- 1664-3224
- Language
- English