Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2 in yeast
2021-02-04 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2 in yeast
Zhang, Y.; De Laurentiis, E.; Bohnsack, K. E.; Wahlig, M.; Ranjan, N.; Gruseck, S. & Hackert, P. et al. (2021)
Nature Communications, 12(1). DOI: https://doi.org/10.1038/s41467-021-20981-3
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Details
- Authors
- Zhang, Ying; De Laurentiis, Evelina; Bohnsack, Katherine E.; Wahlig, Mascha; Ranjan, Namit; Gruseck, Simon; Hackert, Philipp; Wölfle, Tina; Rodnina, Marina V.; Schwappach, Blanche; Rospert, Sabine
- Abstract
- The guided entry of tail-anchored proteins (GET) pathway assists in the posttranslational delivery of tail-anchored proteins, containing a single C-terminal transmembrane domain, to the ER. Here we uncover how the yeast GET pathway component Get4/5 facilitates capture of tail-anchored proteins by Sgt2, which interacts with tail-anchors and hands them over to the targeting component Get3. Get4/5 binds directly and with high affinity to ribosomes, positions Sgt2 close to the ribosomal tunnel exit, and facilitates the capture of tail-anchored proteins by Sgt2. The contact sites of Get4/5 on the ribosome overlap with those of SRP, the factor mediating cotranslational ER-targeting. Exposure of internal transmembrane domains at the tunnel exit induces high-affinity ribosome binding of SRP, which in turn prevents ribosome binding of Get4/5. In this way, the position of a transmembrane domain within nascent ER-targeted proteins mediates partitioning into either the GET or SRP pathway directly at the ribosomal tunnel exit.
- Issue Date
- 4-February-2021
- Journal
- Nature Communications
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente
SFB 1190 | P04: Der GET-Rezeptor als ein Eingangstor zum ER und sein Zusammenspiel mit GET bodies
SFB 1190 | P16: Co-translationaler Einbau von Proteinen in die bakterielle Plasmamembran - Working Group
- RG Schwappach (Membrane Protein Biogenesis)
RG K. Bohnsack (RNA Metabolism)
RG Rodnina - eISSN
- 2041-1723
- Language
- English
- Sponsor
- Deutsche Forschungsgemeinschaft (German Research Foundation) https://doi.org/10.13039/501100001659