A uniform response to mismatches in codon-anticodon complexes ensures ribosomal fidelity
2006 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Gromadski, Kirill B.; Daviter, T; Rodnina, Marina
- Abstract
- Ribosomes take an active part in aminoacyl-tRNA selection by distinguishing correct and incorrect codon-anticodon pairs. Correct codon-anticodon complexes are recognized by a network of ribosome contacts that are specific for each position of the codon-anticodon duplex and involve A-minor RNA interactions. Here, we show by kinetic analysis that single mismatches at any position of the codon-anticodon complex result in slower forward reactions and a uniformly 1000-fold faster dissociation of the tRNA from the ribosome. This suggests that high-fidelity tRNA selection is achieved by a conformational switch of the decoding site between accepting and rejecting modes, regardless of the thermodynamic stability of the respective codon-anticodon complexes or their docking partners at the decoding site. The forward reactions on mismatched codons were particularly sensitive to the disruption of the A-minor interactions with 16S rRNA and determined the variations in the misreading efficiency of near-cognate codons.
- Issue Date
- 2006
- Publisher
- Cell Press
- Journal
- Molecular Cell
- ISSN
- 1097-2765