19 F electron nuclear double resonance (ENDOR) spectroscopy for distance measurements using trityl spin labels in DNA duplexes
2023 | journal article. A publication with affiliation to the University of Göttingen.
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19 F electron nuclear double resonance (ENDOR) spectroscopy for distance measurements using trityl spin labels in DNA duplexes
Asanbaeva, N. B.; Novopashina, D. S.; Rogozhnikova, O. Y.; Tormyshev, V. M.; Kehl, A.; Sukhanov, A. A. & Shernyukov, A. V. et al. (2023)
Physical Chemistry, Chemical Physics, 25(35) pp. 23454-23466. DOI: https://doi.org/10.1039/D3CP02969G
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Details
- Authors
- Asanbaeva, N. B.; Novopashina, D. S.; Rogozhnikova, O. Yu.; Tormyshev, V. M.; Kehl, A.; Sukhanov, A. A.; Shernyukov, A. V.; Genaev, A. M.; Lomzov, A. A.; Bennati, M.; Bagryanskaya, E. G.
- Abstract
- The combination of fluorine labeling and pulsed electron-nuclear double resonance (ENDOR) is emerging as a powerful technique for obtaining structural information about proteins and nucleic acids.
The combination of fluorine labeling and pulsed electron-nuclear double resonance (ENDOR) is emerging as a powerful technique for obtaining structural information about proteins and nucleic acids. In this work, we explored the capability of Mims 19 F ENDOR experiments on reporting intermolecular distances in trityl- and 19 F-labeled DNA duplexes at three electron paramagnetic resonance (EPR) frequencies (34, 94, and 263 GHz). For spin labeling, we used the hydrophobic Finland trityl radical and hydrophilic OX063 trityl radical. Fluorine labels were introduced into two positions of a DNA oligonucleotide. The results indicated that hyperfine splittings visible in the ENDOR spectra are consistent with the most populated interspin distances between 19 F and the trityl radical predicted from molecular dynamic (MD) simulations. Moreover, for some cases, ENDOR spectral simulations based on MD results were able to reproduce the conformational distribution reflected in the experimental ENDOR line broadening. Additionally, MD simulations provided more detailed information about the melting of terminal base pairs of the oligonucleotides and about the configuration of the trityls relative to a DNA end. - Issue Date
- 2023
- Journal
- Physical Chemistry, Chemical Physics
- ISSN
- 1463-9076
- eISSN
- 1463-9084
- Language
- English
- Sponsor
- Russian Science Foundation https://doi.org/10.13039/501100006769