Lipid vesicle pools studied by passive X-ray microrheology
2023 | journal article. A publication with affiliation to the University of Göttingen.
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Lipid vesicle pools studied by passive X-ray microrheology
Czajka, T.; Neuhaus, C.; Alfken, J.; Stammer, M.; Chushkin, Y.; Pontoni, D. & Hoffmann, C. et al. (2023)
The European Physical Journal E, 46(12). DOI: https://doi.org/10.1140/epje/s10189-023-00375-7
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- Authors
- Czajka, Titus; Neuhaus, Charlotte; Alfken, Jette; Stammer, Moritz; Chushkin, Yuriy; Pontoni, Diego; Hoffmann, Christian; Milovanovic, Dragomir; Salditt, Tim
- Abstract
- Abstract Vesicle pools can form by attractive interaction in a solution, mediated by proteins or divalent ions such as calcium. The pools, which are alternatively also denoted as vesicle clusters, form by liquid-liquid phase separation (LLPS) from an initially homogeneous solution. Due to the short range liquid-like order of vesicles in the pool or cluster, the vesicle-rich phase can also be regarded as a condensate, and one would like to better understand not only the structure of these systems, but also their dynamics. The diffusion of vesicles, in particular, is expected to change when vesicles are arrested in a pool. Here we investigate whether passive microrheology based on X-ray photon correlation spectroscopy (XPCS) is a suitable tool to study model systems of artificial lipid vesicles exhibiting LLPS, and more generally also other heterogeneous biomolecular fluids. We show that by adding highly scattering tracer particles to the solution, valuable information on the single vesicle as well as collective dynamics can be inferred. While the correlation functions reveal freely diffusing tracer particles in solutions at low CaCl $_{2}$ 2 concentrations, the relaxation rate $\Gamma (q)$ Γ ( q ) shows a nonlinear dependence on $q^2$ q 2 at a higher concentration of around 8 mM CaCl $_{2}$ 2 , characterised by two linear regimes with a broad cross-over. We explain this finding based on arrested diffusion in percolating vesicle clusters. Graphic Abstract
- Issue Date
- 2023
- Journal
- The European Physical Journal E
- Project
- SFB 1286: Quantitative Synaptologie
SFB 1286 | A02: Bestimmung der Struktur synaptischer Organellen durch Röntgenbeugungs- und Bildgebungsverfahren
SFB 1456: Mathematik des Experiments: Die Herausforderung indirekter Messungen in den Naturwissenschaften
SFB 1456 | Cluster C | C03: Intensity correlations in diffraction experiments: convolution, reconstruction and information - Working Group
- RG Milovanovic (Molecular Neuroscience)
RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics) - ISSN
- 1292-8941
- eISSN
- 1292-895X
- Language
- English