Structure of the multi-subunit chloroplast RNA polymerase
2024-03-07 | journal article
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Structure of the multi-subunit chloroplast RNA polymerase
do Prado, P. F. V.; Ahrens, F. M.; Liebers, M.; Ditz, N.; Braun, H.-P.; Pfannschmidt, T. & Hillen, H. S. (2024)
Molecular Cell, 84(5) pp. 910-925.e5. DOI: https://doi.org/10.1016/j.molcel.2024.02.003
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Details
- Authors
- do Prado, Paula F. V.; Ahrens, Frederik M.; Liebers, Monique; Ditz, Noah; Braun, Hans-Peter; Pfannschmidt, Thomas; Hillen, Hauke S.
- Abstract
- Chloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. Here, we report the cryo-EM structure of a 19-subunit PEP complex from Sinapis alba (white mustard). The structure reveals that the PEP core resembles prokaryotic and nuclear RNAPs but contains chloroplast-specific features that mediate interactions with the PAPs. The PAPs are unrelated to known transcription factors and arrange around the core in a unique fashion. Their structures suggest potential functions during transcription in the chemical environment of chloroplasts. These results reveal structural insights into chloroplast transcription and provide a framework for understanding photosynthesis gene expression.
- Issue Date
- 7-March-2024
- Journal
- Molecular Cell
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1565: Molekulare Mechanismen und Vernetzung von Prozessen der Genexpression
SFB 1565 | P13: Mechanismen und Koordination zwischen Transkription und Ribosomenbiogenese in Mitochondrien
FOR 2848: Architektur und Heterogenität der inneren mitochondrialen Membran auf der Nanoskala
FOR 2848 | P10: Strukturelle Grundlage und Organisation der humanen Cytochrom C Oxidase Biogenese - Working Group
- RG Hillen (Structure and Function of Molecular Machines)
- ISSN
- 1097-2765
- eISSN
- 1097-4164
- Language
- English