Kinetic determinants of high-fidelity tRNA discrimination on the ribosome
2004 | journal article; research paper. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Documents & Media
Details
- Authors
- Gromadski, Kirill B.; Rodnina, Marina
- Abstract
- The ribosome selects aminoacyl-tRNA (aa-tRNA) matching to the mRNA codon from the bulk of nonmatching aa-tRNAs in two consecutive selection steps, initial selection and proofreading. Here we report the kinetic analysis of selection taking place under conditions where the overall selectivity was close to values observed in vivo and initial selection and proofreading contributed about equally. Comparison of the rate constants shows that the 350-fold difference in stabilities of cognate and near-cognate codon-anticodon complexes is not used for tRNA selection due to high rate of GTP hydrolysis in the cognate complex. tRNA selection at the initial selection step is entirely kinetically controlled and is due to much faster (650-fold) GTP hydrolysis of cognate compared to near-cognate substrate.
- Issue Date
- 2004
- Publisher
- Cell Press
- Journal
- Molecular Cell
- ISSN
- 1097-2765