Immunofluorescence-based screening identifies germ cell associated microRNA 302 as an antagonist to p63 expression
2009 | journal article. A publication with affiliation to the University of Göttingen.
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- Authors
- Scheel, Andreas Hans Joachim; Beyer, Ulrike; Agami, Reuven; Dobbelstein, Matthias
- Abstract
- The tumor suppressor homologue p63 is required for proper skin and limb development, but specific isoforms of it also act as a "guardian of the germline." To gain insight into the regulation of p63 expression, we performed immunofluorescence-based screening assays. Using a large collection of microRNA expression plasmids, we identified microRNAs of the 302 cluster as potent suppressors of p63 accumulation in various cell species. MiR-302 reduces p63 protein and mRNA levels through two target sites within the p63 3' untranslated region. In testicular cancer cells, endogenous miR-302 contributes to the suppression of p63. MiR-302 might also contribute to the elimination of p63 in mature oocytes. Thus, miR-302 appears as part of a stringent regulatory mechanism for p63 in germ cells, reminiscent of the tight control for p53 levels in somatic cells.
- Issue Date
- 2009
- Status
- published
- Publisher
- Landes Bioscience
- Journal
- Cell Cycle
- ISSN
- 1538-4101