An elongation factor G-induced ribosome rearrangement precedes tRNA-mRNA translocation
2003 | journal article; research paper. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Documents & Media
Details
- Authors
- Savelsbergh, Andreas; Katunin, Vladimir I.; Mohr, Dagmar; Peske, Frank ; Rodnina, Marina ; Wintermeyer, Wolfgang
- Abstract
- The elongation cycle of protein synthesis is completed by translocation, a rearrangement during which two tRNAs; bound to the mRNA move on the ribosome. The reaction is promoted by elongation factor G (EF-G) and accelerated by GTP hydrolysis. Here we report a pre-steady-state kinetic analysis of translocation. The kinetic model suggests that GTP hydrolysis drives a conformational rearrangement of the ribosome that precedes and limits the rates of tRNA-mRNA translocation and Pi release from EF-G.GDP.Pi. The latter two steps are intrinsically rapid and take place at random. These results indicate that the energy of GTP hydrolysis is utilized to promote the ribosome rearrangement and to bias spontaneous fluctuations within the ribosome-EF-G complex toward unidirectional movement of mRNA and tRNA.
- Issue Date
- 2003
- Publisher
- Cell Press
- Journal
- Molecular Cell
- ISSN
- 1097-2765