The nuclear pore component Nup358 promotes transportin-dependent nuclear import
2009 | journal article. A publication with affiliation to the University of Göttingen.
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The nuclear pore component Nup358 promotes transportin-dependent nuclear import
Hutten, S.; Waelde, S.; Spillner, C.; Hauber, J. & Kehlenbach, R. H. (2009)
Journal of Cell Science, 122(8) pp. 1100-1110. DOI: https://doi.org/10.1242/jcs.040154
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- Authors
- Hutten, Saskia; Waelde, Sarah; Spillner, Christiane; Hauber, Joachim; Kehlenbach, Ralph H.
- Abstract
- Nup358 (also known as RanBP2), a component of the cytoplasmic filaments of the nuclear pore complex, has been implicated in various nucleocytoplasmic transport pathways. Here, we identify Nup358 as an important factor for transportin-mediated nuclear import. Depletion of Nup358 resulted in a strong inhibition of nuclear import of the human immunodeficiency virus type 1 (HIV-1) Rev protein. HIV-1 Rev is an RNA-binding protein that is required for CRM1 ( also known as exportin 1)-dependent nuclear export of unspliced or partially spliced viral RNA. We show that transportin is the major nuclear import receptor for HIV-1 Rev in HeLa cells. Overexpression of transportin strongly promoted nuclear import of HIV-1 Rev in Nup358-depleted cells, indicating that the import receptor becomes rate-limiting under these conditions. Importantly, the import rate of other transportin-dependent proteins was also significantly reduced in Nup358-depleted cells. Our data therefore suggest a general role for Nup358 in transportin-mediated nuclear import.
- Issue Date
- 2009
- Status
- published
- Publisher
- Company Of Biologists Ltd
- Journal
- Journal of Cell Science
- ISSN
- 0021-9533