Mass spectrometric Sequencing of individual peptides from combinatorial libraries via specific generation of chain-terminated sequences
2002 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Mass spectrometric Sequencing of individual peptides from combinatorial libraries via specific generation of chain-terminated sequences
Hoffmann, C.; Blechschmidt, D.; Krüger, R.; Karas, M. & Griesinger, C. (2002)
Journal of Combinatorial Chemistry, 4(1) pp. 79-86. DOI: https://doi.org/10.1021/cc010057x
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- Authors
- Hoffmann, Christian; Blechschmidt, Dierk; Krüger, Ralf; Karas, Michael; Griesinger, Christian
- Abstract
- Combinatorial peptide libraries are a versatile tool for drug discovery. On-bead assays identify reactive peptides by enzyme-catalyzed staining and, usually, sequencing by Edman degradation. Unfortunately, the latter method is expensive and time-consuming and requires free N termini of the peptides. A method of rapid and unambiguous peptide sequencing by utilizing synthesis-implemented generation of termination sequences with subsequent matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometric analysis is introduced here. The required capped sequences are determined and optimized for a specific peptide library by a computer algorithm implemented in the program Biblio. A total of 99.7% of the sequences of a heptapeptide library sample could be decoded utilizing a single bead for each spectrum. To synthesize these libraries, an optimized capping approach has been introduced.
- Issue Date
- 2002
- Journal
- Journal of Combinatorial Chemistry
- ISSN
- 1520-4766
- Language
- English